ATTEMPTS FOR DETECTION OF NANOPARTICLES-NANOBACTERIA AND DISTRIBUTION OF THEIR ANTIBODIES IN SAUDI PATIENTS WITH UROLITHIASIS

Abstract

Calcifying biologic nanoparticles (CNPs) have been identified from diverse tissue samples including kidney stones and calcified aneurysms. Whether or not they represent independent, biologic entities or a form of self-perpetuating biomineralization remains controversial.
In this study, 50 serum samples collected from Saudi patients with urolithiasis and 20 samples collected randomly from healthy individuals were tested for detecting anti- CNP IgG using commercially available ELISA kits. Seven renal stone samples were obtained from National Guard-Health Affairs, King Abdullaziz Medical City, KSA. Each stone sample is divided into two fragments; one fragment was cultured in Dulbecco's Modified Eagle's Medium (DMEM) supplemented with 10% fetal calf serum under cell culture condition to investigate the living nature of CNPs and the other fragment was used for chemical analysis.
Results indicated that 98% of patients have anti-CNP Abs compared with 20% in healthy individuals. Light Microscopy with DIC optic revealed the absence of any microorganism in the tested culture media and no visible mineralized biofilm were observed in cell culture in any of the seven tested stones. Chemically, 71, 4% (5 out of 7) of tested stones were calcium oxalate and 28, 6% (2 out of 7) were urate.