IMPACT OF DIFFERENT MEDIA AND GENOTYPES IN IMPROVING ANTHER CULTURE RESPONSE IN RICE (Oryza sativa) IN BANGLADESH

Abstract

Rice is an important crop in Bangladesh.In vitro production of doubled haploid (DH) plants through anther-culture provides an efficient method for rapid production of homozygous lines. For this purpose, two different media MS and N6 supplemented with 1mg/L BAP +1mg/L 2,4- D+0.5mg/L NAA were evaluated for callus induction and green plant regeneration.Results showed that the highest percentages of calluses and green plants as a continuous process were obtained by N6 compared to MS. The best response to callus formation was obtained by BR48 and and minimum green plant obtained from OM576.